Real-Time PCR
What is Real-Time PCR ?
Real-Time PCR, also known as quantitative PCR (qPCR), is a cutting-edge molecular biology technique used to amplify and quantify DNA or RNA in real time. Unlike conventional PCR, where results are analyzed after the reaction is complete, Real-Time PCR allows for the monitoring of the reaction as it progresses, providing both qualitative and quantitative data.
How Does Real-Time PCR Work?
1 -Sample Preparation : DNA or RNA is extracted from the sample and reverse-transcribed into complementary DNA if working with RNA.
2 -Amplification : The DNA is amplified through a series of thermal cycles using specific primers and probes or dyes.
3 -Fluorescence Detection : As the DNA is amplified, fluorescent dyes or probes emit signals that are detected in real time. The intensity of fluorescence correlates with the amount of DNA being amplified.
Advantages of Real-Time PCR
1 -Quantification:
Real-Time PCR provides precise and accurate quantification of DNA or RNA in a sample.
2 -Sensitivity:
Real-Time PCR can detect even low levels of DNA or RNA, making it ideal for detecting pathogens, genetic mutations, or rare transcripts.
3 -Specificity:
With the use of sequence-specific probes, qPCR ensures high specificity for the target sequence.
Applications of Real-Time PCR.
4 -Disease Diagnosis:
Widely used for detecting infectious diseases (e.g., COVID-19, tuberculosis), cancer biomarkers, and genetic disorders.
Gene Expression Studies:
Helps measure changes in gene expression levels under different conditions.
Pathogen Detection:
Used in food safety, agriculture, and environmental monitoring to identify harmful microorganisms.
Why is Real-Time PCR Important?
Real-Time PCR has revolutionized molecular biology and diagnostics by providing faster, more accurate, and more reliable results. It is now an essential tool in research, medicine, and industry, offering a deeper understanding of genetic and molecular processes.
Looking for Real-Time PCR products or services?
Contact us to learn more about how we can support your research or diagnostic needs with high-quality reagents, advanced instruments, and expert guidance!
